The new rare earth terbium complex synthesis and application
Long-lived rare-earth fluorescence probe based on fluorescence characteristics and developed highly sensitive biochemical analysis of time-resolved fluorescence technology has been in clinical testing and bio-technology has been widely used. In this study, the design, synthesis and characterization of three respectively for the detection of Zn2 +, hydroxyl radical (-OH), H_2O_2 the terbium fluorescence probe; N, N, N1, N1-{2, 6 - bis (3'-aminomethyl -1 '- pyrazolyl) -4 - [N, N-bis (2 - pyridylmethyl) aminomethyl pyridine]} EDTA-Tb3 + (BBATA-Tb3 +), N, N, N1, N1-[2,6 - bis (3'-aminomethyl -1 '- pyrazolyl) -4-- (4'-aminophenoxy) methyl pyridine] EDTA-Tb3 + (BMPTA-Tb3 +) and N, N, N1, N1-[2,6 - bis (3'-aminomethyl -1 '- pyrazolyl) -4-- (3', 4'-diamino phenoxy ) methylene pyridine] EDTA-Tb3 + (BMTA-Tb3 +). Nuclear magnetic resonance, elemental analysis and mass spectrometry by means of synthesis of intermediates and final products were characterized by established based on the detection of Tb complexes Zn2 +, · OH, H202 of the time-resolved fluorescence measurement techniques and time-resolved fluorescence imaging.
Three very weak fluorescence of the probe itself, when after the reaction with the target substance detecting fluorescence quantum yield was significantly enhanced, as strong fluorescent complexes in aqueous solution can be achieved Zn2 +, · OH, H202 high sensitivity detection. Which, BMTA-Tb3 + on the detection of H2O2 in the horseradish peroxidase (HRP) catalyst to achieve. Probe with a fluorescent long life, good water solubility, good stability, high sensitivity, suitable for use under physiological conditions. After the probe is esterified through the thin 'membrane into the cell, combined with time-resolved fluorescent microscopy, which can effectively eliminate from the short-lived background fluorescence and scattered light interference, to achieve a living HeLa cells Zn2 +, · OH, H202 determination. Established target in living cells with high sensitivity detection method.
